The mycelium is the mycelium, the fruiting body of the fungus, made up of fine filaments called hyphae. The mycelium develops from the spores that form on the fruiting body of the fungus. For the needs of mushroom growing, mycelium is produced in mycological laboratories in compliance with sterility during preparation. Now for the cultivation of mushrooms, grain mycelium is used, i.e. grown on grain, but before getting on grain, it goes through the stage of sowing spores and developing a mother culture.
- 30g oat flour (chop the oatmeal)
- 970 ml of water, 15 g of agar or 100 g of gelatin, 2 containers (pots) for cooking the substrate and for a water bath.
- Sterile test tubes and cotton-gauze stoppers for them, foil, inoculation loop (made of knitting needles, wire 100 mm long, bent at the end and reduced to nothing) or scalpel, bactericidal lamp, glass bottles or jars with a capacity of 1 or 3 liters, fine mesh or cardboard for drying grain.
- Ripe mushroom, 10 kg of wheat grain, 15 l of water, a large container for cooking grain, 130 g of gypsum, 30 g of chalk.
Prepare a nutrient medium for spore seeding. Use oatmeal, water, gelatin, or agar. Soak the gelatin with some of the water taken from the volume taken, when it swells, heat it up in a water bath. Boil oatmeal with the remaining water for an hour, filter. Combine oatmeal "jelly" with gelatin (if you took agar, then boil the "jelly" until the agar is completely dissolved).
Pour the liquid gelatin (agar) substrate into test tubes (filling 2/3 of the volume). Close with cotton-gauze plugs. Sterilize for half an hour in a water bath. After sterilization, position the tubes at an angle to increase the area of the substrate, resulting in the so-called beveled substrate.
When the substrate has cooled, take a mature mushroom, cut a piece of lamellar spore-bearing tissue with an inoculation loop or scalpel and place it on the surface of the substrate. Close with the same stopper, wrap with foil on top. Place the tubes in a dark, warm (+ 24 ° C) place. After two weeks, the mycelium will fully grow on the prepared medium and will be ready for further growth. You have prepared a mycelium stock culture. According to various sources, it can be stored at a temperature of + 1 + 2 ° C for 4 to 12 months.
To proceed to the next step and obtain grain mycelium, take one part of grain (wheat, barley) and one and a half parts of water, for example, 10 kg of grain and 15 liters of water. The grain should be boiled over low heat until soft. Time, again, according to various sources, ranges from 15 minutes to 1 hour. This is due to the fact that for mushrooms, the grain should be boiled longer than for oyster mushrooms. Be careful not to boil the grain.
Dry the boiled grain in any available way - on a fine mesh, on a clean towel, on a cardboard, you can use a room fan, - spread it in a layer of 2 - 3 cm.Add 1.3% gypsum and 0.3% chalk to the grain (in our example - 130 and 30 g, respectively).
Fill any glass containers (liter, three-liter jars) with grain at? volume, compact the substrate and make a depression in the center with a diameter of 2.5-3 cm. Roll up the jars with metal lids, having previously made a hole in them with a diameter of 2, 5 - 3 cm. Plug the hole with cotton-gauze plugs and put the jars in the oven to sterilize for 2 hours at a temperature of 120 ° C. Smaller glass containers can be plugged with a cork, wrap the cork on top with foil, put in a saucepan with water and boil for two hours twice, at intervals of a day.
The last thing left is to transplant the uterine culture onto the prepared grain substrate. This demanding procedure also requires sterility. After the jars with grain have cooled to room temperature, you just have to transplant the mother culture into the grain substrate. To do this, take test tubes, slightly heat them over a flame to easily separate the contents from the walls.Use an inoculation loop to retrieve the uterine mycelium. Open the cap of the grain jar and carefully insert the mycelium into the prepared hole. Keeping sterility, close the jars.
After sowing, place the jars with mycelium in a warm, dark place (+ 24 ° C) until they are completely overgrown. The rate of grain overgrowth with mycelium is not the same in different species. The oyster mushroom will master the new substrate in a week, and the champignon will need three times as long. The shelf life of mycelium at temperatures from + 20 ° to + 24 ° C - 24 hours, from + 15 ° to + 18 ° C - 3 days, from 0 to + 2 ° C - 2 weeks, from -2 to 0 - 1 month …